Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Absorbance photometric filter for use with FilterMax family microplate readers. The filter provides a centered transmission at 490 nm with a narrow 8 nm bandwidth to support absorbance-based assays requiring selective 490 nm detection.
- Optimized for absorbance measurements at 490 nm.
- Narrow 8 nm bandwidth for selective detection.
- Designed for installation in compatible microplate reader filter holders.
- Suitable for microplate-based colorimetric and enzyme assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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This polarization emission filter is an accessory for multimode microplate readers, intended for fluorescence polarization (FP) measurements in the visible range. It enables polarization-based fluorescence assays when installed in the reader's emission filter position.
- Enables fluorescence polarization emission measurements.
- Designed for visible wavelength range.
- Intended for multimode microplate readers.
- Supplied as a single unit.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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